Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 5, Pages 2042-2047Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac902791q
Keywords
-
Categories
Funding
- National Institute of Environmental Health Sciences [U01ES016026]
- NSF [CBET-0617240]
- DOD/DARPA/DMEA [DMEA90-02-2-0216]
- U.S. EPA [GR-83237501]
- Conacyt
Ask authors/readers for more resources
We demonstrate a highly sensitive nano aptasensor for anthrax toxin through the detection of its polypeptide entity, protective antigen (PA toxin) using a PA toxin ssDNA aptamer functionalized single-walled carbon nanotubes (SWNTs) device. The aptamer was developed in-house by capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX) and had a dissociation constant (k(d)) of 112 nM. The aptasensor displayed a wide dynamic range spanning up to 800 nM with a detection limit of 1 nM. The sensitivity was 0.11 per nM, and it was reusable six times. The aptasensor was also highly selective for PA toxin with no interference from human and bovine serum albumin, demonstrating it as a potential tool for rapid and point-of-care diagnosis for anthrax.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available