Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 20, Pages 8566-8572Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac101659d
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- National Science Council of Taiwan [NSC 98-2113-M-002-011-MY3, NSC 98-2627-M-002-013, NSC 98-2627-M-002-014]
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We have developed a simple and homogeneous fluorescence assay, comprised of 3-mercaptopropionic acid (MPA) and DNA-Cu/Ag nanoclusters (NCs) in aqueous solution, for the detection of Cu2+ ions. The fluorescence of the DNA-Cu/Ag NCs was quenched by MPA, which was recovered in the presence of Cu2+ ions. This MPA-induced fluorescence quenching arises through changes in the DNA conformation that occur after interactions between MPA and the Cu/Ag clusters. The MPA-induced fluorescence quenching displayed typical characteristics in Stern-Volmer plots; it followed a static quenching mechanism. The presence of Cu2+ ions resulted in the oxidation of MPA to form a disulfide compound, leading to recovery of the fluorescence of the DNA-Cu/Ag NCs. The fluorescence of the DNA-Cu/Ag NCs in the presence of MPA increased upon increasing the concentration of Cu2+ ions over the range from 5 to 200 nM. The DNA-Cu/Ag NC probe provided the limit of detection (at a signal-to-noise ratio of 3) for Cu2+ ions of 2.7 nM, with high selectivity (by at least 2300-fold over other tested metal ions). We validated the practicality of using this probe for the detection of Cu2+ ions in environmental samples through analyses of Montana soil and pond water samples.
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