Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 14, Pages 6163-6167Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac100977b
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Funding
- Natural Sciences and Engineering Research Council of Canada
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Differential mobility spectrometry has been applied to reveal the occurrence of isomerization of thymine nucleobase and of thyrnine dideoxynucleotide d(5'-TT-3') due to bond redisposition induced by UV irradiation at 254 nm of frozen aqueous solutions of these molecules. Collision-induced dissociation (CID) spectra of electro-sprayed photoproducts of the thymine solution suggest the presence of two isomers (the so-called cyclobutane and 6,4-photoproducts) in addition to the proton-bound thymine dimer, and these were separated using differential mobility spectrometry/mass spectrometry (DMS/MS) techniques with water as the modifier. Similar experiments with d(5'-TT-3') revealed the formation of a new isomer of deprotonated thymine dideoxynucleotide upon UV irradiation that was easily distinguished using DMS/MS with isopropanol as the modifier. The results reinforce the usefulness of DMS/MS in isomer separation.
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