Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 12, Pages 5038-5045Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac9028664
Keywords
-
Categories
Funding
- National Institutes of Health [F32-GM083510, R37-GM053757]
Ask authors/readers for more resources
Relative levels of ribosomal proteins were quantified in crude cell lysates using mass spectrometry. A method for quantifying cellular protein levels using macromolecular standards is presented that does not require complex sample separation, identification of high-responding peptides, affinity purification, or postgrowth modifications. Perturbations in ribosomal protein levels by overexpression of individual proteins correlate to known autoregulatory mechanisms and extend the network of ribosomal protein regulation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available