Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 12, Pages 5005-5011Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac1009047
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Funding
- National Natural Science Foundation of China [J0830415, 20975034]
- U.S. Department of Energy [DE-FG02-08ER64568]
- National Institutes of Health [ES016865]
- National Science Foundation [CTS-0120978, DMI-0328162]
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The catalytic beacon has emerged as a general platform for sensing metal ions and organic molecules. However, few reports have taken advantage of the true potential of catalytic beacons in signal amplification through multiple enzymatic turnovers, as existing designs require either equal concentrations of substrate and DNAzyme or an excess of DNAzyme in order to maintain efficient quenching, eliminating the excess of substrate necessary for multiple turnovers. On the basis of the large difference in the melting temperatures between the intramolecular molecular beacon stem and intermolecular products of identical sequences, we here report a general strategy of catalytic and molecular beacon (CAMB) that combines the advantages of the molecular beacon for highly efficient quenching with the catalytic beacon for amplified sensing through enzymatic turnovers. Such a CAMB design allows detection of metal ions such as Pb(2+) with a high sensitivity (LOD = 600 pM). Furthermore, the aptamer sequence has been introduced into DNAzyrne to use the modified CAMB for amplified sensing of adenosine with similar high sensitivity. These results together demonstrate that CAMB provides a general platform for amplified detection of a wide range of targets.
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