Journal
ANALYTICAL CHEMISTRY
Volume 82, Issue 5, Pages 1905-1910Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac9027356
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Funding
- National Cancer Institute [R01CA119402]
- SAIC-Frederick [28XS119]
- NIH
- Center for Nanotechnology
- IGERT [DGF-0504573]
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Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded micro-channels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO2 surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
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