Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 2, Pages 834-839Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac8024015
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Funding
- French Agence Nationale de la Recherche [ANR-05-PNANO-035-01, ANR-07-NANO-006-03]
- National Science Foundation [ECS-0622225, ECS-0637121]
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The fluorescence enhancement found in gold nanoapertures is demonstrated to increase the signal-to-noise ratio (SNR) in fluorescence correlation spectroscopy (FCS). Starting from a general discussion on noise in FCS experiments, we show that fluorescence enhancement leads to a dramatic increase in the SNR. This prediction is confirmed by experiments where we report an experimental gain in SNR of about 1 order of magnitude, corresponding to a 100-fold reduction of the experiment duration. This technique is then applied to monitor the kinetics of a fast enzymatic cleavage reaction. This set of experiments evidence the feasibility of FCS analysis with fast integration times of about 1 s, opening the way to the monitoring of a variety of biochemical reactions at reduced time scales.
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