Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 22, Pages 9507-9511Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac901965v
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Funding
- Defense Advanced Research [HR0011-06-1-0050]
- National Science Foundation [CBET-0729771-002]
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We present and demonstrate a novel technique for the purification of nucleic acids from biological samples using isotachophoresis (ITP). We demonstrate a simple and rapid method to achieve ITP-based extraction, preconcentration, and purification of DNA from nanoliter volumes of whole blood. We show that ITP purification yields genomic DNA samples which can be quantitated with fluorescence measurements and are immediately compatible with polymerase chain reaction (PCR) (e.g., a PCR-friendly solution free of significant inhibitors). We hypothesize ITP purification is applicable to processing of a wide range of complex biological samples.
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