Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 9, Pages 3329-3333Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac900395x
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Funding
- National Natural Science Foundation [20573041, 20773044, 20873050]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0422]
- Program for New Century Excellent Talents in University [1306009]
- Development Program of the Science and Technology of Jilin Province [20060902-02]
- KAK-ENHI [470, 20043032]
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- China Scholarship Council
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We detected concentration-dependent surface-enhanced Raman scattering (SERS) spectra of several label-free proteins (lysozyme, ribonuclease B, avidin, catalase, and hemoglobin) for the first time in aqueous solutions. Acidified sulfate was used as an aggregation agent to induce high electromagnetic enhancement in SERS. Strong SERS spectra of simple and conjugated protein samples could easily be accessed after the pretreatment with the aggregation agent. The detection limits of the proposed method for lysozyme and catalase were as low as 5 mu g/ mL and 50 ng/mL, respectively. This detection protocol for label-free proteins has combined simplicity, sensitivity, and reproducibility and allows routine qualitative and relatively quantitative detections. Thus, it has great potential in practical high-throughput protein detections.
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