Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 12, Pages 4813-4821Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac900403z
Keywords
-
Categories
Funding
- Ministere de l'Enseignement Superieur et de la Recherche
- Centre National de la Recherche Scientifique (CNRS)
- Agence National de la Recherche (ANIZ) [ANR-05-BLAN-0397]
- Fondation d'Entreprise EADS
- Conseil Scientifique de l'Universite Louis Pasteur
- Medical Research Council (U.K.)
- Ministry of Defence (U.K.)
- EC FP6 Marie Curie Research Training Network
- ProSA
- Human Frontier Science Program (HFSP)
- EMBO [ALTF 915-2006]
- Agence Nationale de la Recherche (ANR) [ANR-05-BLAN-0397] Funding Source: Agence Nationale de la Recherche (ANR)
Ask authors/readers for more resources
We have developed a method for high-throughput isothermal amplification of single DNA molecules in a droplet-based microfluidic system. DNA amplification in droplets was analyzed using an intercalating fluorochrome, allowing fast and accurate digital quantification of the template DNA based on the Poisson distribution of DNA molecules in droplets. The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
