Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 16, Pages 7102-7106Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac901234r
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Funding
- NIH/NINDS [1R21NS061051]
- NIH [5 T32 CA073479-09]
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Gaussia luciferase (Glue) is a sensitive reporter for studying different biological processes such as gene expression, promoter activity, protein-protein interactions, signal transduction, as well as tumor cell growth and response to therapy. Since Glue is naturally secreted, the kinetics of these processes can be monitored in real-time by measuring an aliquot of conditioned medium in culture or a few microliters of blood in vivo at different time points. Glue catalyzes light emission with a short half-life which is unfavorable for certain applications. We isolated a Glue mutant that catalyzes enhanced light stability in the presence of a detergent, in combination with high sensitivity, making it an attractive luciferase for high-throughput functional screening applications.
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