Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 15, Pages 6503-6508Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac900923e
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Effective systems for rapid, sequence-specific nucleic acid detection at the point of care would be valuable for a wide variety of applications, including clinical diagnostics, food safety, forensics, and environmental monitoring. Electrochemical detection offers many advantages as a basis for such platforms, including portability and ready integration with electronics. Toward this end, we report the Integrated Microfluidic Electrochemical DNA (IMED) sensor, which combines three key biochemical functionalities-symmetric PCR, enzymatic single-stranded DNA generation, and sequence-specific electrochemical detection-in a disposable, monolithic chip. Using this platform, we demonstrate detection of genomic DNA from Salmonella enterica serovar Typhimurium LT2 with a limit of detection of < 10 aM, which is similar to 2 orders of magnitude lower than that from previously reported electrochemical chip-based methods.
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