Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 2, Pages 840-844Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac801235b
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Funding
- Grant Agency of Academy of Sciences of the Czech Republic [A100040602, A400040804]
- Grant Agency of the Czech Republic [301/07/0490]
- Polish Ministry of Science and Higher Education [1 T09A 121 30]
- Institute of Biophysics [AV0Z50040702, AVOZ50040507]
- Institute of Medical Biology Polish Academy of Sciences
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Polyhedral boron clusters are proposed as new, chemically and biologically stable, versatile redox labels for electrochemical DNA hybridization sensors. Selective and sensitive detection of the redox labeled DNA-probe was achieved by means of covalently attached electroactive marker 7,8-dicarba-nido-undekaborate group. A nanomolar concentration of boron cluster-labeled DNA was recognized. High specificity of the analysis with the boron cluster-labeled DNA probe, including detection of single-base mismatch, was demonstrated. The above findings, together with proposed earlier use of metallacarboranes as an electrochemical label for biomolecules opens the door for a multicolor electrochemical coding of DNA with boron clusters and simultaneous detection of several DNA targets.
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