Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 23, Pages 9585-9589Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac901518a
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Funding
- National Institute on Drug Abuse, NIH
- Office of National Drug Control Policy (ONDCP)
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In previous papers, we highlighted the role of ammonium sulfate in increasing peptide fragmentation by in-source decay (ISD). The current work systematically investigated effects of matrix assisted laser desorption ionization (MALDI) extraction delay, peptide amino acid composition, matrix, and ammonium sulfate concentration on peptide ISD fragmentation. The data confirmed that ammonium sulfate increased peptides signal-to-noise ratio as well as their in-source fragmentation, resulting in complete sequence coverage regardless of the amino acid composition. This method is easy, inexpensive, and generates the peptides sequence instantly.
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