Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 3, Pages 1016-1022Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac802068n
Keywords
-
Categories
Funding
- University of Texas at San Antonio
- National Institute of General Medical Sciences (NIGMS)/National Institutes of Health [1SC3GM081085]
Ask authors/readers for more resources
We have investigated the interaction Of D-amino acid oxidase (DAAO) with single-walled carbon nanotubes (CNT) by spectroscopic ellipsometry. Dynamic adsorption experiments were performed at different experimental conditions. In addition, the activity of the enzyme adsorbed at different conditions was studied. Our results indicate that DAAO can be adsorbed to CNT at different pH values and concentrations by a combination of hydrophobic and electrostatic interactions. Considering that the highest enzymatic activity was obtained by adsorbing the protein at pH 5.7 and 0.1 mg.mL(-1), our results indicate that DAAO can adopt multiple orientations on the surface, which are ultimately responsible for significant differences in catalytic activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available