Chromatically Resolved Optical Microscope (CROMoscope): A Grating-Based Instrument for Spectral Imaging

The chromatically resolved optical microscope (CROMoscope) is capable of spectral imaging with tunable spectral and spatial resolutions. Because of its remarkably simple design, the CROMoscope can be easily assembled and aligned. Spectral resolution as low as 2.5 nm full width at half maximum (fwhm) was measured using an atomic emission line of Hg. Absorption spectra of different parts of a micrograph can readily be compiled using white-light illumination. Chloroplast absorption from an Elodea plant leaf was used to demonstrate this capability. Spectral imaging is widely applicable to many areas of science, and the CROMoscope is particularly simple to adapt to conventional microscopes and should enable detailed spectroscopic information to be obtained from microscopy.