Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 16, Pages 7123-7126Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac901126d
Keywords
-
Categories
Funding
- American Diabetes Association [7-08-RA-191]
- Cleveland State University (Research Challenge Award)
Ask authors/readers for more resources
Voltage-controlled amplification of the output current of an enzymatic detector has been demonstrated. By application of an external voltage between the gating electrode and the working electrode on which the enzyme glucose oxidase was immobilized, the biocatalytic output current of the detector was increased significantly, allowing the detection limit of glucose to be lowered from the millimolar level to the picomolar level. The current amplification could be reversibly controlled by the applied voltage. Application of this technique to the ethanol-alcohol dehydrogenase system showed similar results. The detection setup suggests that the output current is controlled by the electric field at the interface between the solution and the working electrode. The enzyme's biospecificity was preserved in the presence of the field. The detector, with its output current controlled by a voltage applied at a third electrode, behaves as a field-effect transistor, whose current-generating mechanism is the conversion of an analyte to a product using an enzyme as catalyst. In a broader sense, the operation of the detector shows a means for manipulating a redox enzymatic reaction.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available