Journal
ANALYTICAL CHEMISTRY
Volume 81, Issue 11, Pages 4414-4421Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac9002672
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Funding
- NIH/National Institute of Biomedical Imaging and Bioengineering (NIBIB) [R21EB008247]
- Nanoscale Science and Engineering Center [EEC-0425626]
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R21EB008247] Funding Source: NIH RePORTER
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We have recently developed a semicontinuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. With the use of pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10-15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and Joule heating in SFE.
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