Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 18, Pages 7075-7083Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac800610z
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Funding
- New Century Excellent Talents in Fujian Province [Z03131]
- Natural Science Foundation of China [20701031]
- Natural Science Foundation of Fujian Province of China [C0710045]
- Xiamen University [200602]
- Fujian Province for Young Scientific Researchers, China [2006F3128]
- [2006CB933300]
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An electrochemical biosensor using a boron-doped diamond (BDD) electrode is described for differentiating between gene sequences according to DNA hybridization events using an ac impedimetric approach. BDD electrodes were dipped into a 1% solution of polyethylenimine (PEI) to adsorb a thin layer of positively charged PEI on the surface of BDD, then PEI-modified BDD electrodes were used to immobilize negatively charged single-stranded PCR fragments from Exon 7 of human p53 gene. Alternating current impedimetric measurements were first performed on these systems in phosphate buffered saline (PBS) and then upon exposure to single-stranded DNA (ssDNA). When the ssDNA-immobilized BDD electrode and solution ssDNA were completely complementary, a large drop in impedance was measured. Complementary DNA could be clearly detected at concentrations down to 10(-19) g mL(-1) at a fixed frequency (10 Hz). Higher concentrations of DNA gave faster hybridization with saturation occurring at levels above 1.0 pg mL(-1). Responses were much lower upon exposure to noncDNA, even at higher concentrations. The results show it is possible to directly detect target DNA at a fixed frequency and without additional labeling.
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