Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 8, Pages 3025-3028Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac702637w
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Funding
- NCI NIH HHS [R21 CA122648, 5R21CA122648] Funding Source: Medline
- NIGMS NIH HHS [R01 GM066137, R01 GM079359, 5R01GM066137] Funding Source: Medline
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Real time intracellular monitoring of biological molecules inside living cells is important in many biomedical studies and reveals valuable information unobtainable by conventional molecular biology techniques. A variety of methods and molecular probes have been developed, but long term (from a few hours to days) intracellular monitoring with high sensitivity and selectivity is impossible and has not been accomplished. We have used locked nucleic acids (LNA) to engineer novel molecular beacons (MBs) for long-term intracellular monitoring. The LNAMBs were made of a mixed LNA and DNA bases to have extremely high biostability. The new beacons were tested with MDA-MB-231 cancer cells and used effectively to monitor mRNA expression levels in real-time for 5-24 h. After 24 h inside living cells, the LNA-MBs were still functional, demonstrating a greatly enhanced stability enabling the measurement of intracellular gene expression over an extended period of time.
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