Journal
BIOCONJUGATE CHEMISTRY
Volume 18, Issue 4, Pages 1318-1324Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc070080x
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Funding
- NIGMS NIH HHS [R21 GM078447, R21 GM078447-02, R21GM078447, R21 GM078447-01] Funding Source: Medline
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A 21-mer peptide that can be used to covalently introduce synthetic molecules into proteins has been developed. Phage-displayed peptide libraries were subjected to reaction-based selection with 1,3-diketones. The peptide was further evolved by addition of a randomized region and reselection for improved binding. The resulting 21-mer peptide had a reactive amino group that formed an enaminone with 1,3-diketone and was used as a tag for labeling of maltose binding protein. Using this peptide tag and 1,3-diketone derivatives, a variety of molecules such as reporter probes and functionalities may be covalently introduced into proteins of interest.
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