Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 45, Issue 1, Pages 42-46Publisher
WILEY
DOI: 10.1111/j.1472-765X.2007.02145.x
Keywords
impedimetric method; Pseudomonas aeruginosa; selective synthetic culture medium
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Aims: To investigate whether the use of a novel synthetic medium in conjunction with impedimetric technology could provide a rapid and automated detection of Pseudomonas aeruginosa in water samples. Methods and Results: A selective synthetic medium (Z-broth) in which the only carbon and nitrogen source is acetamide was applied in direct impedimetric examination for the selective isolation of P. aeruginosa. A total of 1036 tap-water, well-water, swimming-pool water and dialysis water samples were investigated, and any P. aeruginosa contamination was detected in 7-24 h. Neither false-negative nor false-positive results were observed. Conclusions: The results of the present evaluation demonstrate that impedance measurement with the use of Z-broth is suitable for the rapid and automatic detection of P. aeruginosa in water. Significance and Impact of the Study: The main advantages of the method: 240 samples can be examined in one step, the procedure is fully automated, the results are obtained quickly and the labour and media requirements are low.
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