Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 70, Issue 1, Pages 209-213Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2007.03.018
Keywords
Pseudomonas; hydrogen cyanide (HCN); diversity
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A PCR-based assay targeting hcnAB, essential genes for hydrogen cyanide (HCN) biosynthesis, allowed sensitive detection of HCN+ pseudomonads between logs 2.9 and 3.5 cells per PCR reaction tube. RFLP analysis revealed 13 allele combinations among selected 2,4-diacetylphloroglucinol-producing (Phl(+))HCN+, and 13 alleles in Phl(-) HCN+ strains from a global collection. (C) 2007 Elsevier B.V. All rights reserved.
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