Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 1, Pages 143-149Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac701647s
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Funding
- NATIONAL CANCER INSTITUTE [R21CA126191] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR018522] Funding Source: NIH RePORTER
- NCI NIH HHS [R21 CA126191-01, R21 CA126191] Funding Source: Medline
- NCRR NIH HHS [RR018522, P41 RR018522-05, P41 RR018522] Funding Source: Medline
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We describe the coupling of liquid chromatography (LC) separations with mass spectrometry (MS) using nanoelectrospray ionization (nano-ESI) multiemitters. The array of 19 emitters reduced the flow rate delivered to each emitter, allowing the enhanced sensitivity that is characteristic of nano-ESI to be extended to higher flow rate separations. The signal for tryptic fragments from proteins spiked into a human plasma sample increased 11-fold on average when the multiemitters were employed, due to increased ionization efficiency and improved ion transfer efficiency through a newly designed heated multicapillary MS inlet Additionally, the LC peak signal-to-noise ratio increased similar to 7-fold when the multiemitter configuration was used. The low dead volume of the emitter arrays preserved peak shape and resolution for robust capillary LC separations using total flow rates of 2 mu L/min.
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