Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 23, Pages 9029-9033Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac801424y
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Funding
- National Natural Science Foundation [20873175, 20725516]
- Shanghai Municipal Commission for Science and Technology [0752nm021, 06ZR14106, 06ZR14136]
- Ministry of Science adn Technology of China [2006CB933000, 2007CB936000, 2007AA06A406]
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In this work, we report a novel electrochemical DNA sensor based on a nonfouling monolayer structure self-assembled at gold surfaces. Self-assembled monolayers (SAMs) with oligo(ethylene glycol) (OEG)-terminated thiols are known to be highly protein-resistant and effectively repel nonspecific adsorption. We found that a mixed SAM structure incorporating thiolated oligonucleotides and OEG thiols (SH-DNA/OEG) exhibited the similar nonfouling feature. More importantly, it allowed facile electron transfer across the monolayer and thus was fully compatible with electrochemical detection. On the basis of this SH-DNA/OEG platform, we developed a sandwich-type electrochemical sensor for the sequence-specific detection of DNA targets. This sensor was able to detect as little as 1 pM target DNA even in the presence of complicated biological fluids such as human serum. We also employed this sensor to directly detect a polymerase chain reaction (PCR) amplicon from the genomic DNA of Escherichia coli K12, which led to a very low detect limit of 60 fg (similar to 10 copies).
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