Journal
NANO LETTERS
Volume 7, Issue 7, Pages 2043-2045Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl0709120
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Funding
- NIAMS NIH HHS [AR44420] Funding Source: Medline
- NIGMS NIH HHS [GM68625] Funding Source: Medline
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We introduce a bifocal imaging method that enables three-dimensional (3D) tracking of both fluorescent and nonfluorescent particles. We accomplish this by simultaneously imaging a focused plane, for in-plane position (x,y), and a defocused plane, for out-of-plane position (z), of a molecule using a single CCD camera. We applied our method to several systems including in vivo melanosome tracking and phagocytosed fluorescent bead tracking. We have achieved 2-5 nm accuracy with a 2-50 ms time resolution.
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