Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 10, Pages 3824-3831Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac8000786
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Funding
- NCRR NIH HHS [RR018522, P41 RR018522, P41 RR018522-06] Funding Source: Medline
- NIAID NIH HHS [Y1-AI-4894-01NIH] Funding Source: Medline
- NIBIB NIH HHS [P41 EB002503] Funding Source: Medline
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Despite widespread interest in combining laboratory-on-a-chip technologies with mass spectrometry (MS)-based analyses, the coupling of microfluidics to electrospray ionization (ESI)-MS remains challenging. We report a robust, integrated poly(dimethylsiloxane) microchip interface for ESI-MS using simple and widely accessible microfabrication procedures. The interface uses an auxiliary channel to provide electrical contact for the stable cone-jet electrospray without sample loss or dilution. The electric field at the channel terminus is enhanced by two vertical cuts that cause the interface to taper to a line rather than to a point, and the formation of a small Taylor cone at the channel exit ensures subnanoliter postcolumn dead volumes. Cone-jet mode electrospray was demonstrated for up to 90% aqueous solutions and for extended durations. Comparable ESI-MS sensitivities were achieved using both microchip and conventional fused silica capillary emitters, but stable cone-jet mode electrosprays could be established over a far broader range of flow rates (from 50-1000 nL/min) and applied potentials using the microchip emitters. This attribute of the microchip emitter should simplify electrospray optimization and make the stable electrospray more resistant to external perturbations.
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