Journal
REPRODUCTIVE SCIENCES
Volume 14, Issue 5, Pages 475-485Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1933719107305035
Keywords
human nonmetastatic clone 23 type 1 gene; fungal immunomodulatory protein Ganoderma tsugae; cervical cancer cells; cell migration; cell invasion; matrix metalloproteinase
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The authors investigate the effects of human nonmetastatic clone 23 type 1 (nm23-H1) gene and fungal immunomodulatory protein-Ganoderma tsugae (FIP-gts) on the metastatic potential of cervical cancer cells and assess whether nm23-H1 can influence the action of FIP-gts using cell migration and invasion assays and gelatin zymography. The nm23-H1 gene was stably transfected into Caski cells, which lacked nm23-H1 expression, The results show that nm23-H1 stably transfected Caski cells exhibit reduced cell migration but no change of cell invasion and matrix metalloproteinase (MMP)-2 and -9 activities. FIP-gts reduced cell migration in SiHa and nm23-H1 transfected Caski cells more significantly compared with Caski cells and reduced invasion in Caski and nm23-H1-transfected Caski cells, but it exerted no influence on MMP-2 and MMP-9 activities in them. Conclusively, the nm23-H1 gene suppresses cervical cancer cell migration but not invasion and activities of MMP-2 and MMP-9 and enhances the inhibition of FIP-gts upon migration.
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