Journal
OSTEOARTHRITIS AND CARTILAGE
Volume 15, Issue 7, Pages 780-788Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2007.01.007
Keywords
cartilage; collagen; anisotropy; FTIRI; PLM; microscopy; histological zones
Categories
Funding
- NIAMS NIH HHS [R01 AR045172-07, R01 AR045172-02, R01 AR045172-06A1, R01 AR045172, R01 AR 45172, R01 AR045172-08] Funding Source: Medline
Ask authors/readers for more resources
Objective: To study the anisotropic characteristics of individual histological zones in articular cartilage using Fourier-transform infrared imaging (FTIRI) at 6.25 mu m pixel resolution. Method: A canine humeral cartilage-bone block was paraffin-embedded and microtomed into 6 mu m sections. Each of the five sections was infrared (IR)-imaged 26 times with identical acquisition parameters, for a 5-10 degrees increment of a wire grid polarizer introduced before the detector in 0-180 degrees angular space. Following the IR imaging experiments, the same tissue sections were also imaged by polarized light microscopy (PLM). Results: The IR absorption components of cartilage (amide I, amide II, amide III, and sugar) exhibit distinctly different anisotropies, which vary differently as a function of the tissue depth. A new type of image, the absorbance anisotropy map, was constructed for each major component, which shows that (1) the absorbance of the amide components in most parts of the tissue is anisotropic, (2) the anisotropic behavior in the radial and the superficial zones of the tissue is opposite, (3) the absorption profile of amide I is inverse to those of amide II and amide III, and (4) the IR absorption of the sugar component is almost isotropic. The anisotropic variations of the amide components were fitted to an empirical equation. Conclusions: The IR anisotropy map is a powerful tool to monitor the individual chemical components in articular cartilage. The ability to examine the same tissue section using both FTIRI and PLM offers the possibility of correlating the tissue's morphology with chemical distribution. (c) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available