Journal
ANALYTICAL CHEMISTRY
Volume 80, Issue 18, Pages 7063-7067Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac801199k
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Funding
- EPSRC
- RCUK Basic Technology Programme
- Engineering and Physical Sciences Research Council [EP/D048664/1] Funding Source: researchfish
- EPSRC [EP/D048664/1] Funding Source: UKRI
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Rapid kinetic measurements are important in understanding chemical interactions especially for biological molecules. Herein, we present a droplet-based microfluidic platform to study streptavidin-biotin binding kinetics with millisecond time resolution. With integration of a confocal fluorescence detection system, individual droplets can be monitored and characterized online to extract kinetic information. Using this approach, binding kinetics between streptavidin and biotin were observed via fluorescence resonance energy transfer. The binding rate constant of streptavidin and biotin was found to be in a range of 3.0 x 10(6)-4.5 x 10(7) M-1 S-1.
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