Journal
ANALYTICAL BIOCHEMISTRY
Volume 458, Issue -, Pages 43-48Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2014.04.015
Keywords
Flow cytometry; Silver nanoparticles; Plasmon resonance; Light scattering
Funding
- Department of Energy [DE-FG02-06ER46342]
- National Institutes of Health - United States [1R01GM081040]
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Silver nanoparticles have been modified with self-assembled monolayers of hydroxyl-terminated long chain thiols and encapsulated with a silica shell. The resulting core shell nanoparticles were used as optical labels for cell analysis using flow cytometly and microscopy. The excitation of plasmon resonances in nanoparticles results in strong depolarized scattering of visible light, permitting detection at the single nanoparticle level. The nanoparticles were modified with neutravidin via epoxide azide coupling chemistry, to which biotinylated antibodies targeting cell surface receptors were bound. The nanoparticle labels exhibited long-term stability in solutions with high salt concentrations without aggregation or silver etching. Labeled cells exhibited two orders of magnitude enhancement of the scattering intensity compared with unlabeled cells. (c) 2014 Elsevier Inc. All rights reserved.
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