Journal
ANALYTICAL BIOCHEMISTRY
Volume 437, Issue 2, Pages 161-163Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.02.007
Keywords
Microfluidic; Bioreactor; Perfusion; Flow rate; Dermal fibroblasts
Funding
- Korea-New Zealand Joint Research Program
- Ministry of Education, Science, and Technology [2011K000700]
- Industrial Strategic Technology Development Program [10035197]
- Ministry of Knowledge Economy of Korea
- National Research Foundation of Korea [2011-0031321] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The current research engineered a pumpless energy-efficient microfluidic perfusion cell culture chip that works by modifying the basic gravity-driven siphon flow using an intravenous (IV) infusion set as a conventional, inexpensive, and sterile tool. The IV set was modified to control the constant hydrostatic head difference, thereby maintaining the steady flow rate medium perfusion. The micro-bioreactor chip demonstrated flexibility in controlling a wide range of flow rates from 0.1 to 10 ml/min, among which 1- and 5-ml/min flow rates were examined as suitable shear flows for long-term dermal fibroblast cell culture, paving the way for artificial skin development. (C) 2013 Elsevier Inc. All rights reserved.
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