Journal
ANALYTICAL BIOCHEMISTRY
Volume 433, Issue 2, Pages 150-152Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.10.023
Keywords
Block copolymer; Capillary electrophoresis; DNA; PEG; PNA; SNP
Funding
- Strategic Programs for RD at RIKEN
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [20245020]
- Grants-in-Aid for Scientific Research [20245020] Funding Source: KAKEN
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To estimate allele frequency of single-nucleotide polymorphisms (SNPs) in pooled DNAs with secondary structures, an affinity capillary electrophoresis was developed using an allele-specific peptide nucleic acid probe modified with polyethylene glycol. This probe disrupted secondary structures of DNA analytes and hybridized to them during electrophoresis. Such DNA-binding capability allowed separation of the folded analytes with a single-base difference within 20 min. The feed ratio of the target allele was evaluated by calculating the peak area ratio. The averaged difference between the feed and observed ratio was 1.5%. This method should be of general applicability to quantitative SNP analyses. (C) 2012 Elsevier Inc. All rights reserved.
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