Journal
ANALYTICAL BIOCHEMISTRY
Volume 440, Issue 1, Pages 18-22Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.04.026
Keywords
Maize chlorotic mottle virus; Surface plasmon resonance; Biosensor; Virus detection
Funding
- important National Science and Technology specific projects [2013ZX08012-001]
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We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with a dynamic range from I to 1000 ppb. The detection limit is approximately 1 ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly specific recognition for both purified MCMV and crude extracts from real-world samples. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
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