Journal
ANALYTICAL BIOCHEMISTRY
Volume 443, Issue 2, Pages 169-171Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.09.004
Keywords
Poly(amido amine) dendrimer; Platinum nanoparticle; Multiwalled carbon nanotube; Enzyme reaction; Electrochemical biosensor; Inhibition reaction
Funding
- National Taiwan University of Science and Technology, Taiwan [100H451201]
- National Taiwan University of Science and Technology, Taiwan
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The presented approach provides the advanced development of effective, rapid, and versatile electrochemical sensors for a small amount of analytes on potential, cheap, and disposable printed chips. The electrocatalytic activity of this biosensor revealed the feasible detection of hydrogen peroxide at low potential (similar to 0.09 V) and the detection of a biocontaminant inhibitor (organophosphorus pesticide) in a wide range of concentrations. This efficiency comes from the chemical immobilization of catalysts (Pt nanoparticles) and electron transfer-enlarging materials (carbon nanotubes) on an electrode. Especially, dendrimers raise the stable conjugation of enzymes (acetylcholinesterase/choline oxidase/peroxidase) as well as nanoparticles and carbon nanotubes on an electrode. (C) 2013 Elsevier Inc. All rights reserved.
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