Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 129, Issue 26, Pages 8068-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja072013j
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Funding
- NIBIB NIH HHS [R01 EB001991-09, EB001991, R01 EB001991] Funding Source: Medline
- NIGMS NIH HHS [R29 GM055843, GM55843, R01 GM055843, R01 GM055843-13] Funding Source: Medline
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In this paper, we describe the efficient synthesis of a diazirine cross-linker (photo-Met) and demonstrate that this amino acid is compatible with solid-phase peptide synthesis and expressed protein ligation (EPL). Accordingly, we have exploited the unique power of semi-synthesis to incorporate multiple different probes into the same protein by preparing a version of Smad2 signaling protein containing both photo-Met and an activating phospho-serine. Through judicious placement of the cross-linker, we have been able to covalently capture a transient MH2-MH2 interaction that is dependent upon the PTM in the protein.
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