Journal
ANALYTICAL BIOCHEMISTRY
Volume 442, Issue 2, Pages 249-252Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2013.07.030
Keywords
Salicylic acid; Magnetic nanoparticles; Genomic DNA; Extraction; Simple and rapid
Funding
- W.M. Keck Foundation
- Indiana Clinical and Translational Sciences Institute
- National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award [TR000006]
Ask authors/readers for more resources
Salicylic acid-coated magnetic nanoparticles were prepared via a modified one-step synthesis and used for a one-stop extraction of genomic DNA from mammalian cells. The synthesized magnetic particles were used for magnetic separation of cells from the media by nonspecific binding of the particles as well as extraction of genomic DNA from the lysate. The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reaction. The entire process of extraction and isolation can be completed within 30 min. Compared with traditional methods based on centrifugation and filtration, the established method is fast, simple, reliable, and environmentally friendly. (C) 2013 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available