Journal
ANALYTICAL BIOCHEMISTRY
Volume 434, Issue 1, Pages 199-201Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.11.009
Keywords
MiRNA; Nanoengineered gold shells; LDI-TOF MS; Self-assembled monolayers
Funding
- Basic Science Research Program [2009-0064280]
- Priority Research Centers Program through the National Research Foundation of Korea [2012-0006686]
- Ministry of Education, Science, and Technology
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We report on a simple, fast, and highly sensitive method that allows femtomolar detection of microRNAs (miRNAs) without the need of polymerase chain reaction using a nanoengineered micro gold shell (mu AuS) and laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS). Target miRNAs are selectively captured by a chemically decorated gold chip and a mu AuS, which carries with it a large number of small molecules, termed amplification tags (Am-tags). Subsequent LDI-TOF MS analysis allows a highly amplified mass signal of Am-tags for the presence of target miRNAs in a sample, resulting in ultrasensitive detection. (C) 2012 Elsevier Inc. All rights reserved.
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