Journal
ANALYTICAL BIOCHEMISTRY
Volume 434, Issue 1, Pages 187-198Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.10.035
Keywords
Ionic matrices; Pre-spotted MALDI plates; MALDI; Quantification; Drugs; Peptidomic; MALDI MSI
Funding
- Ministere de l'Education Nationale, de L'Enseignement Superieur et de la Recherche
- Region Nord-Pas de Calais
- Universite Lille 1
- ANR FunMALDI
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In the current study, we compared plastic matrix-assisted laser desorption/ionization (MALDI) plates pre-spotted with different solid ionic matrices. Data reflect that after 3 months of storage, the standards were oxidized in alpha-cyano-4-hydroxycinnamic acid (HCCA) whether or not in HCCA/3-acetylpyridine (3APY) and HCCA/aniline, and certain peptides, such as ubiquitin, were not detected using the HCCA matrix, whereas they were detected in pre-spotted ionic matrices. Application in peptidomics of these MALDI matrices pre-spotted plates (after 3 months of storage) with ovarian cyst fluid showed less intense signals with HCCA than with solid ionic matrices. We show that these pre-spotted ionic matrices plates can be used for relative drug quantification, high mass protein detection, and MALDI mass spectrometry imaging. (c) 2012 Elsevier Inc. All rights reserved.
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