Journal
ANALYTICAL BIOCHEMISTRY
Volume 425, Issue 1, Pages 88-90Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.03.005
Keywords
3T3-L1 cells; Adipocyte differentiation; Rosiglitazone
Funding
- Deutsche Forschungsgemeinschaft [BR 2430/4-3]
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In this note, we present a detailed procedure for highly effective and reproducible 313-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, 313-L1 cells are widely used for studying adipogenesis and the biochemistry of adipocytes. However, using different kits and protocols published so far, we were not able to obtain full differentiation of the currently available American Type Culture Collection (ATCC) 313-L1 cell lots. Using rosiglitazone (2 mu M) as an additional prodifferentiative agent, we achieved apparently complete differentiation of 313-L1 cells within 10 to 12 days that persisted for at least up to cell culture passage 10. (C) 2012 Elsevier Inc. All rights reserved.
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