Journal
ANALYTICAL BIOCHEMISTRY
Volume 423, Issue 2, Pages 195-201Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.01.026
Keywords
Aptamer; PCB77; SELEX; Gold nanoparticles; Fluorescence
Funding
- National Basic Research Program of China [2007CB936602]
- National 863 Research Program [2008AA062504]
- National Natural Science Foundation of China [20977093, 10935009, 10225526]
- Chinese Academy of Sciences
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Polychlorinated biphenyls (PCBs) have been of major concerns for decades due to their potential toxicity to human health. To trace the PCBs efficiently and sensitively, many detection methods have been developed. Aptamers, a new class of diagnostic tools, are considered to be such additional candidates for detection of pollutants. In the current study, we report the DNA aptamers, isolated by FluMag-SELEX (a modified SELEX [systematic evolution of ligands by exponential enrichment] technology), that recognize PCBs with the dissociation constants (K-d values) down to the micromolar range. Using the selected aptamers, a highly sensitive aptamer-based fluorescent assay for detection of PCBs was established using gold nanoparticles, with a widely linear range from 0.1 to 100 ng/ml. Moreover, our aptamer-based gold nanoprobe displays specificity toward 3,3',4,4'-tetrachlorobiphenyl (PCB77) compared with a few common PCB77 structural analogs. These results open the possibility of using aptamers as biorecognition elements for easy and fast environmental monitoring. (C) 2012 Elsevier Inc. All rights reserved.
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