Journal
ANALYTICAL BIOCHEMISTRY
Volume 424, Issue 2, Pages 124-126Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.02.015
Keywords
Endotoxin removing; Plasmid; Triton X-114
Funding
- National Natural Science Foundation of China [30873161, 81001448]
- Foundation of Shaanxi Educational Committee of China [2010JS099]
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Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method. (c) 2012 Elsevier Inc. All rights reserved.
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