Journal
FEBS LETTERS
Volume 581, Issue 17, Pages 3157-3163Publisher
WILEY
DOI: 10.1016/j.febslet.2007.05.081
Keywords
elovl; polyunsaturated fatty acid; fatty acid; triacylglycerol; lipogenesis; elongation
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Elongation of very long-chain fatty acids (ELOVL) members were overexpressed in two preadipocyte cell lines, ELOVL2 and ELOVL3 in 3T3-L1 cells, and ELOVL1-3 in F442A cells. Cells overexpressing ELOVL2, whose preferred substrates are arachidonic acid (AA, C20:4n-6) and eicosapentaenoic acid (EPA, C20:5n-3), showed an enhanced triacylglycerol (TAG) synthesis and subsequent accumulation of lipid droplets. Incorporation of fatty acid (FA) but not of glucose into TAG was enhanced by ELOVL2-overexpression. Two lipogenic genes encoding diacylglycerol acyltransferase-2 (DGAT2) and fatty acid-binding protein-4 (FABP4, aP2) were induced in ELOVL2-overexpressing cells, whereas no such effect was seen on the fatty acid synthase (FAS) gene. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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