Journal
ANALYTICAL BIOCHEMISTRY
Volume 426, Issue 2, Pages 91-93Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2012.04.013
Keywords
Reuse; Regeneration; Oligonucleotide; Microarray; Comparative genomic hybridization; Fluorescent labeling; Dimethylurea
Funding
- Foundation for Polish Science [FOCUS 4/2008, FOCUS-GRANTS 4/08/2009]
- Polpharma Scientific Foundation
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Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4 Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200 kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA. (C) 2012 Elsevier Inc. All rights reserved.
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