Journal
ANALYTICAL BIOCHEMISTRY
Volume 421, Issue 1, Pages 339-341Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.10.033
Keywords
Concanavalin A-magnetic particle conjugates; Glycoproteomics; Native cell membrane glycoproteins; Mass spectrometry
Funding
- National Natural Science Foundation of China [30870549]
- Chinese Ministry of Science and Technology [2009DFA32730]
Ask authors/readers for more resources
The discovery, isolation, and subsequent identification of cell membrane glycoproteins involved in the structure and function of the cell surface are becoming more and more important. Here, concanavalin A-magnetic particle conjugates were employed to isolate the special membrane glycoproteins from living HepG-2 cells. The isolated glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry as well as annotated. A total of 37 membrane glycoproteins were identified, and 25 of them were ascertained to locate in the extracellular region. (C) 2011 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available