Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1156, Issue 1-2, Pages 87-93Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.10.053
Keywords
HILIC; LC-NMR-MS; hyphenation; endogenous metabolites; body fluids; metabonomics
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A method for the unambiguous identification of highly polar molecules based on the separation on a silica gel column run in hydrophilic interaction chromatography (HILIC) mode followed by mass spectroscopic (MS) analysis and subsequent measurement by nuclear magnetic resonance (NMR) spectroscopy is described. Polar neutral, acidic and basic compounds of small molecular size usually not retained on reversed phase stationary phases can be separated and unequivocally identified by means of MS and NMR spectroscopy. The method is applied to exemplify the identification of the endogenous urine. (c) 2006 Elsevier B.V. All rights reserved.
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