Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 117, Issue 3, Pages 312-318Publisher
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2007.05.003
Keywords
Listeria monocytogenes; internalin A; ready-to-cat; seafood
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InIA is a surface protein participating in the entry of Listeria monocytogenes into mammalian non-phagocytic cells. PrfA is a positive regulatory factor that regulates the expression of a set of virulence genes. Recent studies revealed that some L. monocytogenes strains have a truncated form of these proteins because of nonsense mutations in their sequences, and these truncations contribute to the significant reduction in virulence of this pathogen. In this study, sequence analyses of inIA and prfA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated MIA and all had non-mutated prfA. This indicated that these strains could be fully virulent based on the sizes of these proteins. (C) 2007 Elsevier B.V. All rights reserved.
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