Journal
ANALYTICAL BIOCHEMISTRY
Volume 419, Issue 2, Pages 168-172Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.08.005
Keywords
DNA; Electrochemical; Cyclodextrins; PdS nanoparticle; Molecular recognition
Funding
- JiangXi University of Traditional Chinese Medicine of China
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In this paper, we constructed a new electrochemical biosensor for DNA detection based on a molecule recognition technique. In this sensing protocol, a novel dual-labeled DNA probe (DLP) in a stem-loop structure was employed, which was designed with dabcyl labeled at the 3' end as a guest molecule, and with a Pb nanoparticle labeled at the 5' end as electrochemical tag to indicate hybridization. One alpha-cyclodextrin-modified electrode (alpha-CD/MCNT/GCE) was used for capturing the DNA hybridization. Initially, the DLP was in the closed state in the absence of the target, which shielded dabcyl from the bulky -CD/MCNT/GCE conjugate due to a steric effect. After hybridization, the loop sequence (16 bases) formed a rigid duplex with the target, breaking the relatively shorter stem duplex (6 bases). Consequently, dabcyl was forced away from the Pb nanoparticle and became accessible by the electrode. Therefore, the target hybridization event can be sensitively transduced via detecting the electrochemical reduction current signal of Pb. Using this method, as low as 7.1 x 10(-10) M DNA target had been detected with excellent differentiation ability for even a single mismatch. (C) 2011 Elsevier Inc. All rights reserved.
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