Journal
ANALYTICAL BIOCHEMISTRY
Volume 415, Issue 1, Pages 59-66Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2011.04.003
Keywords
Heparin; Heparan sulfate; Heparin lyase; Disaccharide analysis; Biological samples; Ultra-performance liquid chromatography; Mass spectrometry
Funding
- National Institutes of Health [GM38060, HL096972, HL101721, GM090127]
- Bioengineered Heparin Consortium
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A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities. (C) 2011 Elsevier Inc. All rights reserved.
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