Journal
ANALYTICAL BIOCHEMISTRY
Volume 410, Issue 1, Pages 92-97Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2010.11.019
Keywords
Cytosensing; Electrochemistry; Carbon nanotubes; Cell-surface glycan; Carbohydrates; Lectin
Funding
- National Science Funds for Creative Research Groups [20821063]
- National Natural Science Foundation of China [90713015, 20875044]
- National Basic Research Program of China [2010CB732400]
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A kind of concanavalin A functionalized multiwalled carbon nanotube (ConA-MWCNT) was constructed by noncovalent assembly of ConA on carboxylated MWCNT with poly(diallyldimethylammonium) as a linker. The novel nanomaterial was characterized with scanning electron microscopy and atomic force microscopy. It incorporated both the specific recognition ability of lectin for cell-surface mannosyl groups and the unique electronic and mechanical properties of MWCNT. An electrochemical label-free method for cytosensing was proposed by constructing a ConA-MWCNT interface on a glassy carbon electrode, which showed a linear response to K562 cells ranging from 1 x 10(4) to 1 x 10(7) cells mL(-1). The ConA-MWCNT interface could be further used for monitoring of dynamic variation of glycan expression on K562 cells in response to drugs. A facile and high-throughput optical method for the analysis of dynamic glycan expression on living cells was also developed by constructing an array of ConA-MWCNT spots on a glass slide. This method showed acceptable rapidity and low cost. The noncovalent functionalization of MWCNTs with lectins could be potentially applied in cell biological studies based on cell-surface glycan expression. (C) 2010 Elsevier Inc. All rights reserved.
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